Repairs are completed employing only real Agilent components that match your instrument and improve general performance.
Know your column: Columns have unique pore sizes and solvent tolerances. Test Along with the company with the column to circumvent loading incompatible samples and solvents.
Right design and style of columns delivers elevated resolution involving peaks facilitates the packing process of numerous resin types at distinct bed heights, and offers scalability from system growth to entire-scale manufacturing.
Make certain that the trouble is certainly the column. Samples also can clog autosampler parts, which includes needles and needle seats. To check When your column is certainly the situation, disconnect the column and monitor the tension.
Have got a finite lifetime and in many cases just after good cleanse-up and storage they are going to get to the end of their life time. However, you will discover specified actions which you could adhere to for getting the most life out of one's column.
Be part of Sartorius as we take a look at how to transfer a standalone batch mAb chromatography course of action into a connected DSP.
Resolute® BioSC Pilot can be a multi-step chromatography click here system which can repeatedly function three chromatography separations (in batch or multi-column method), which include viral inactivation and in-line buffer planning. The chaining of various device functions with each other leads to a compact and intensified process.
Intuitive process that will help you choose the correct chromatography column on your biopharmaceutical applications.
Retention factor (kappa primary) measures how long a component of your mixture trapped to the column, measured by the realm underneath the curve of its peak within a chromatogram (because HPLC chromatograms undoubtedly are a function of time).
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Pumps differ in stress capability, but their efficiency is calculated on their capability to generate a steady and reproducible volumetric move level.
Collectively the aspects are variables in a very resolution equation, which describes how perfectly two components' peaks divided or overlapped one another. These parameters are mostly only useful for describing HPLC reversed stage and HPLC standard phase separations, due to the fact People separations are typically additional refined than other HPLC modes (e.g., ion Trade and size exclusion).
The cell section carries a liquid sample through the column for the detector, and compounds or analytes different due to various degrees of interaction While using the stationary period.
We also offer you On Desire repairs in the event of an incident, fix sections and supplies for in-home use, and preventive upkeep programs to create your laboratory a far more successful facility.